Improved hybridization conditions for DNA ‘fingerprints’ probed with M13
نویسندگان
چکیده
منابع مشابه
Improved method for the production of M13 phage and single-stranded DNA for DNA sequencing.
An improved method is described for the efficient production of M13 phage and M13 single-stranded (ss)DNA in a relatively short time period. Infection of E. coli (F') cells with as few as 5 phage particles can yield 10(12) phage particles/mL in 3 hours if the cells are grown in LB broth or SOB broth supplemented with about 5 mM Mg2+. The method tolerates large variations in the initial multipli...
متن کاملA complex of single-strand binding protein and M13 DNA as hybridization probe.
Single-stranded DNA was complexed to the single-strand binding protein (SSB) of Escherichia coli in a mass ratio of 30:1. The protein moiety of this complex can be labelled by a number of methods of which we have chosen radio-iodination and biotinylation as examples. The SSB-M13 DNA complexes, labelled to high specific activities, were used as probes in hybridization experiments in which 1.6 X ...
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We compared the efficiency of transformation of recombinant M13 DNA obtained using the traditional calcium chloride method with that obtained by electroporatlon. M13 cloning experiments are often hampered by low yields I.e. insufficient numbers of recombinant plaques are generated. Low concentration of the target DNA, poor ligation due to target DNA impurity and small target fragment size are c...
متن کاملImproved immobilization of DNA to microwell plates for DNA-DNA hybridization.
An improved and simplified protocol for DNA immobilization was developed to enhance DNA-DNA hybridization on microwell plates. Target DNA was immobilized by simple dry-adsorption. Efficiencies of DNA immobilization and retention were enhanced 1.4-6.5 times and 4.2-19.6 times, respectively, compared with a conventional method. The overall hybridization efficiency was increased 3.1-5.2 times. Thi...
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Latent Overlapped fingerprints are frequently encountered in latent fingerprints lifted from crime scenes. Overlapping occurs when the same location of an object is touched by one or more fingers several times. It is necessary to separate such latent overlapped fingerprints into component fingerprints so that existing fingerprint matchers can recognize them. In fact, fingerprint examiners usual...
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ژورنال
عنوان ژورنال: Nucleic Acids Research
سال: 1988
ISSN: 0305-1048,1362-4962
DOI: 10.1093/nar/16.9.4161